Agdia , Inc. is pleased to herald the launch of a rapid molecular diagnostic psychometric test for the detecting of Dahlia mosaic computer virus ( DMV ) and Dahlia plebeian mosaic virus ( DCMV ) . In add-on to the do - it - yourself ( DIY ) tryout kit , detection of these pathogen is now offered as a service in Agdia ’s Testing Services section for customers who bid to relegate works sample to an ISO 17025 - accredited laboratory .
DMV is the causal agent of dahlia mosaic disease and a phallus of the Caulimovirus genus . In addition to DMV , a 2nd Caulimovirus , DCMV , was key out in direct association with disease symptomology . Like DMV , DCMV is a round , double - stranded DNA computer virus . Neither virus is limited to a geographic region , and both pathogen are far-flung in cultivated dahlia , with mixed infections being vulgar .
Transmission of both virus occurs via mechanically skillful means ( grafting , propagation cuttings , etc . ) . Furthermore , both virus are carry in a non - persistent or semi - persistent manner by aphid after feed on infected plant .
nervure - glade due to Dahlia mosaic virus ( Photo cater courtesy of Claudia Nischwitz and Melanie Stock , Utah State University )
Symptoms vary by cultivar but typically admit random chlorophyl loss ( greensickness ) , nervure clearing , necrotic lesion , leafage deformation , and general stunting . stunt of the plant life often stamp down or prohibits flowering , furnish plants unmarketable . Some cultivars can parade meek symptoms or stay symptomless indefinitely . Early and precise sensing of DMV and DCMV is paramount to the direction of these viruses throughout Dahlia pinnata cultivation .
Agdia ’s raw AmplifyRP ® XRT check for detection of DMV and DCMV is based on recombinase polymerase amplification ( RPA ) . This technology further the speedy amplification and detection of nucleic acid target , DNA or RNA while maintain a individual operating temperature of 39–42 ° ascorbic acid . The AmplifyRP ® XRT Cartesian product achieve sensitivity and specificity comparable to PCR while having clear advantages over the lab - establish technology . AmplifyRP ® XRT products do not command a nucleic acid purification step ; crude sampling extracts are prepare using a wide-eyed descent buffer and tested directly . When paired with Agdia ’s AmpliFire ® isothermal fluorometer , the XRT arrangement is a substance abuser - friendly pecker that can be apply in the area or the lab by force with modified experience in molecular nosology . Total assay time is less than 30 minute when used with the AmpliFire ® as a existent - time assay .
Agdia express their new check was validated against a diverse venire of DMV / DCMV isolate from multiple geographical realm , find all unfeigned positives . sensitiveness for this assay is comparable to that observe with bring out formal PCR , qPCR , and LAMP assay to which it was equate . what is more , this product exhibited a high level of exclusivity against a comprehensive control panel of likely hybridization reactors , let in Carnation etched ring virus , Cauliflower mosaic computer virus , Chrysanthemum stunt virusoid , Cucumber mosaic virus , Dahlia latent viroid , Impatiens Necrotic Spot Virus , Potato virus Y , Rudbeckia flower torture virus , Tobacco streak virus , and Tomato Spotted Wilt Virus . This assay does not detect the endogenic pararetroviral element ( DMV - D10 ) . This assay include an endogenous interior DNA ascendance and was develop to be used with flower , foliage , petiole , root , seminal fluid , fore , and tuber tissue .
For more information : AgdiaRobert Emmitt[email protected][email protected]www.agdia.com
